The Dry Lab Stuff

Data plotting can be easily done in excel. Excel is a very easy and efficient tool for calculations and plotting of biological data and most people including me prefer it. With excel one has to plot the data and do all customization each and every time for a new data set. Therefore, when it comes to plotting multiple datasets of similar nature over and over again, using a programming language is more efficient. Once a template code for a plot is ready, one can plot any number of data sets with it in a few seconds. Here we will see how to plot a simple scatter plot by taking an example of growth profile (i.e. data of time vs O.D.) of a cell culture. The reading are from three experiments. The O.D.s were taken from 0 to 6 hours at an interval of one hour.

Dictionaries are one of the built-in data types in Python. Like the language dictionary has words and their meaning written in it, the python dictionaries have 'key' and the 'value' corresponding to the 'key' stored in it. The python dictionary can be created by writing key-value pairs inside a curly bracket {}. An empty dictionary can be initiated as follows: d = {} Also, we can directly create a dictionary with initial key-value pairs. The key- value pairs must be in the format {key: value} . Following is an example. d = {'A': 3, 'Animals': ['dog', 'lion']} Here, we added two key-value pairs, {'A': 3} and { 'Animals': ['dog', 'lion']}. Note that the values can be a number, a string, a list or even a dictionary. They keys of the dictionary, however, should be an immutable objects such as strings, numbers, and tuples. Add new item to a dictionary New items in a dictionary can be added t...

Printing the values of variables is required. It may serve as an output for user to read or for the developers who are debugging their code. Sometimes we need the printed output to be easier to understand and not just a set of variable values . Using formatted string literals or f-strings is by far the most intuitive way to print out variables along with intermittent text. The simple example of printing using f-strings is as follows: a = 2 b = 3 print(f'The value of a is {a}, and the value of b is {b}.') The value of a is 2 and the value of b is 3.

ANOVA (Analysis of variance) is an statistical methods used when we want to see if some treatment or strategy has produced any significant effect on the outcome or not. One-way ANOVA is used when only one factor is being tested. Two-way ANOVA is used when two factors are being tested at once. For the ANOVA, something called as a F-value is calculated by the following formula: F v a l u e = S S B S S W where, SSB = sum of squares between groups SSW = sum of squares within groups Also, based on the degrees of freedom within and between the groups, a F-critical value and a P-value is obtained. If the calculated F-value is greater than the F-critical value it is inferred that the treatment of strategy has statistically significant effect on the outcome. The P value is the probability of F-values to be greater than or equal to the calculated F-value if there was no effect o...